吴琼


1967年4月生，理学博士，教授、博导。哈尔滨工业大学生命科学与技术学院发育生物学学科带头人，中国动物学会细胞及分子显微技术学分会理事，黑龙江省细胞生物学会秘书长。主要从事干细胞与发育生物学的研究工作（干细胞的诱导分化，基因组的印记机制，非编码RNAs的功能解析，环境荷尔蒙与表观遗传的关系研究及对胚胎发育影响的评价）。1992年毕业于哈尔滨医科大学临床医学专业，并于1996年到日本留学深造。得到造血机制和冬眠机制研究很有造诣的森和博教授和杉本健吉副教授的指导。在他们的研究室从事相关理论与实验的研究达6年之久。参加过多项日本文部省的研究课题，独立完成了冬眠基因的克隆和冬眠机制的研究课题，合作完成了造血机制的研究课题。于2000年获得新泻大学的硕士学位，2003年获得了日本国立新泻大学的理学博士学位。2003年在国际上著名的河野教授的动物发育工学研究室，承担了“生殖细胞的印记机制解明和孤雌生殖动物的开发”的课题的分子生物部分的工作。该课题是日本生研机构的4.5亿日元的课题，为期4年半。该课题组在发育生物学的研究方面取得了令世界注目的成绩，在世界上首次成功的制作出了孤雌发育鼠（Kaguya）。成果发表在2004年4月22日出版的英国《Nature》杂志上。科研成果清楚了孤雌发育胚胎的出生需要正常表达两组imprinted基因（Igf2-H19和Dlk1-Gtl2包括Dio3, Mirg），论文发表在国际知名杂志《Reproduction》上。同时，证明肝脏的发育障碍及肝脏的异常造血是雌核发生胚胎的早期死亡和增长延迟的一个重要的原因。该课题组通过去除小鼠7号染色体上的H19基因的转写单位和DMR区；同时去除小鼠12号染色体上的IG-DMR区而构建的双敲除孤雌发育胚胎，进一步把孤雌发育胚胎的出生率和存活率由0.5%提高到40%，成果发表在了2007年8月19日的《Nature Biotechnology》上。2006年3月开始被哈尔滨工业大学生命科学与工程系聘为教授，从事发育生物学领域的研究工作。承担研究生的高级发育生物学等教学工作。以学术带头人获得哈尔滨工业大学海外引进人才科研启动基金的资助。近年来，研究成果连续发表在《FEBS Letters》(IF=3.341)、《Journal of Molecular Histology》（IF=1.979）、《Nucleic Acids Research》(IF=8.808)、《Development》(IF=6.273)、Nature旗下的《Scientific Reports》(IF=5.078)、《Molecular Genetics and Genomics》（IF=2.831）等期刊。现主持国家自然基金，省自然基金和教育部归国留学人员启动基金及参与国家863等项目的科研工作。先后发表50多篇学术论文，其中SCI论文总影响因子达到100以上，单篇他引达到268次。

近期发表的有关论文:

  1. Liu H*, Liu X, Zhang S, Lv J, Li S, Shang S, Jia S, Wei Y, Wang F, Su J, Wu Q, Zhang Y*. Systematic identification and annotation of human methylation marks based on bisulfite sequencing methylomes reveals distinct roles of cell type-specific hypomethylation in the regulation of cell identity genes. Nucleic Acids Res, 2015,  Dec 3. pii: gkv1332. [Epub ahead of print] PubMed PMID: 26635396.
  2. Wei Y, Zhang S, Shang S, Zhang B, Li S, Wang X, Wang F, Su J, Wu Q, Liu H*, Zhang Y*. SEA: a super-enhancer archive. Nucleic Acids Res. 2015,  Nov 17. pii:gkv1243. [Epub ahead of print] PubMed PMID: 26578594.
  3. Gu N*, Hu H, Guo Q, Jin S, Wang C, Oh Y, Feng Y, Wu Q. Effects of oral administration of titanium dioxide fine-sized particles on plasma glucose in
    mice. Food Chem Toxicol. 2015 Dec;86:124-31. doi: 10.1016/j.fct.2015.10.003. Epub 2015 Oct 22. PMID:26472183.
  4. Gu N*, Guo Q, Mao K, Hu H, Jin S, Zhou Y, He H, Oh Y, Liu C, Wu Q. Palmitate increases musclin gene expression through activation of PERK signaling pathway in C2C12 myotubes. Biochem Biophys Res Commun. 2015 Nov 20;467(3):521-6. doi: 10.1016/j.bbrc.2015.10.005. Epub 2015 Oct 9. PMID:26449458.
  5. Guo J, He H, Liu Q, Zhang F, Lv J, Zeng T, Gu N, Wu Q*. Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct. Mol Cells. 2015 Oct
    31;38(10):859-65. doi: 10.14348/molcells.2015.0098. Epub 2015 Oct 12. PMID: 26447138
  6. Han Z, Yu C, Tian Y, Zeng T, Cui W, Mager J, Wu Q. Expression patterns of long noncoding RNAs from Dlk1-Dio3 imprinted region and the potential mechanisms of Gtl2 activation during blastocyst development.2015 Jul 31;463(3):167-73. doi: 10.1016/j.bbrc.2015.04.126. PMID: 26005002.
  7. Hu H, Guo Q, Wang C, Ma X, He H, Oh Y, Feng Y, Wu Q, Gu N. Titanium dioxide nanoparticles increase plasma glucose via reactive oxygen species-induced insulin resistance in mice. J Appl Toxicol.2015 Oct;35(10):1122-32. doi: 10.1002/jat.3150. PMID: 25826740.
  8. Cui W, Huang Z, He H, Gu N, Qin G, Lv J, Zheng T, Sugimoto K, Wu Q. MiR-1188 at the imprinted Dlk1-Dio3 domain acts as a tumor suppressor in hepatoma cells. Mol Biol Cell.2015 Apr 15;26(8):1416-27. PMID: 25694452
  9. Yan D, Zhang D, Liu H, Wei Y, Lv J, Wang F, Zhang C, Wu Q, Su J, Zhang Y. Chromatin modifications and genomic contexts linked to dynamic DNA methylation patterns across human cell types. Sci Rep. 2015 Feb 12;5:8410. PMID: 25673498
  10. Lv J, Liu H, Liu H, Wu Q, Zhang Y. Genetics and epigenetics in human cancer. Cancer Genetics and Epigenetics. 2014 Dec 31;2(1):1-14.
  11. Zeng TB, He HJ, Han ZB, Zhang FW, Huang ZJ, Liu Q, Cui W, Wu Q. DNA methylation dynamics of a maternally methylated DMR in the mouse Dlk1-Dio3 domain. FEBS Lett. 2014 Dec 20;588(24):4665-71.
  12. Lv J, Liu H, Yu S, Liu H, Cui W, Gao Y, Zheng T, Qin G, Guo J, Zeng T, Han Z, Zhang Y, Wu Q. Identification of 4438 novel lincRNAs involved in mouse pre-implantation embryonic development. Mol Genet Genomics. 2014 Nov 27.
  13. Xiu Y, Liu Z, Xia S, Jin C, Yin H, Zhao W, Wu Q. MicroRNA-137 upregulation increases bladder cancer cell proliferation and invasion by targeting PAQR3. PLoS One. 2014 Oct 16;9(10):e109734.
  14. Lv J, Huang Z, Liu H, Liu H, Cui W, Li B, He H, Guo J, Liu Q, Zhang Y, Wu Q. Identification and characterization of long intergenic non-coding RNAs related to mouse liver development. Mol Genet Genomics. 2014 Dec;289(6):1225-35.
  15. Hu H, Li L, Wang C, He H, Mao K, Ma X, Shi R, Oh Y, Zhang F, Lu Y, Wu Q, Gu N. 4-Phenylbutyric acid increases GLUT4 gene expression through suppression of HDAC5 but not endoplasmic reticulum stress. Cell Physiol Biochem. 2014;33(6):1899-910.
  16. Wei L, Liu C, Kang L, Liu Y, Shi S, Wu Q, Li Y. Experimental study on effect of simulated microgravity on structural chromosome instability of human peripheral blood lymphocytes. PLoS One. 2014 Jun 25;9(6):e100595.
  17. Wei Y, Su J, Liu H, Lv J, Wang F, Yan H, Wen Y, Liu H, Wu Q, Zhang Y. MetaImprint: an information repository of mammalian imprinted genes. Development. 2014 Jun;141(12):2516-23.
  18. Henan X, Toyota N, Yanjiang X, Fujita Y, Zhijun H, Touma M, Qiong W, Sugimoto K. Enhancement of phagocytosis and cytotoxicity in macrophages by tumor-derived IL-18 stimulation. BMB Rep. 2014 May;47(5):286-91.
  19. Lv J, Liu H, Liu H, Wu Q, Zhang Y. Long Non-coding RNAs: key players in brain and central nervous system development. Computational Molecular Biology. 2014 Apr; 4(4):1-13.
  20. Liu H, Wang T, Liu H, Wei Y, Zhao G, Su J, Wu Q, Qiao H, Zhang Y. Detection of type 2 diabetes related modules and genes based on epigenetic networks. BMC Syst Biol. 2014;8 Suppl 1:S5.
  21. Liu H, Zhu R, Lv J, He H, Yang L, Huang Z, Su J, Zhang Y, Yu S, Wu Q. DevMouse, the mouse developmental methylome database and analysis tools. Database (Oxford). 2014 Jan 9;2014:bat084.
  22. Liu Q, He H, Zeng T, Huang Z, Fan T, Wu Q. Neural-specific expression of miR-344-3p during mouse embryonic development. J Mol Histol. 2014 Aug;45(4):363-72.
  23. Lv J, Liu H, Wu Q, Zhang Y. Detection of candidate melanoma blood biomarkers by RNA-Sequencing. Cancer Genetics and Epigenetics. 2013 Dec, 1(2):1-8
  24. Huang Z, Han Z, Cui W, Zhang F, He H, Zeng T, Sugimoto K, Wu Q. Dynamic expression pattern of Pde4d and its relationship with CpG methylation in the promoter during mouse embryo development. Biochem Biophys Res Commun. 2013 Nov 29;441(4):982-7.
  25. Huang Z, Han Z, Zhang F, He H, Yu S, Wu Q. Spatiotemporal expression of retrogene-host pair Mcts2/H13 in mouse embryo, and Mcts2 has no influence on H13 transcription pattern in NIH/3T3 cells. Acta Histochem. 2014 Mar;116(2):312-8.
  26. Lv J, Liu H, Liu H, Wu Q, Zhang Y. Predicting Long Non-coding RNAs Based on Genomic Sequence Information. Computational Molecular Biology. 2013 Dec; 3(4):24-30.
  27. Lv J, Liu H, Huang Z, Su J, He H, Xiu Y, Zhang Y, Wu Q. Long non-coding RNA identification over mouse brain development by integrative modeling of chromatin and genomic features. Nucleic Acids Res. 2013 Dec;41(22):10044-61.
  28. Cai J, Yang L, He HJ, Xu T, Liu HB, Wu Q, Ma Y, Liu QH, Nie MH. Antioxidant capacity responsible for a hypocholesterolemia is independent of dietary cholesterol in adult rats fed rice protein. Gene. 2014 Jan 1;533(1):57-66.
  29. Liu H, Chen Y, Lv J, Liu H, Zhu R, Su J, Liu X, Zhang Y, Wu Q. Quantitative epigenetic co-variation in CpG islands and co-regulation of developmental genes. Sci Rep. 2013;3:2576.
  30. Zhang Y, Su J, Yu D, Wu Q, Yan H. EpiDiff: entropy-based quantitative identification of differential epigenetic modification regions from epigenomes. Conf Proc IEEE Eng Med Biol Soc. 2013;2013:655-8.
  31. Lv J, Cui W, Liu H, He H, Xiu Y, Guo J, Liu H, Liu Q, Zeng T, Chen Y, Zhang Y, Wu Q. Identification and characterization of long non-coding RNAs related to mouse embryonic brain development from available transcriptomic data. PLoS One. 2013 Aug 14;8(8):e71152.
  32. Zeng TB, He HJ, Zhang FW, Han ZB, Huang ZJ, Liu Q, Wu Q. Expression analysis of AK003491, an imprinted noncoding RNA, during mouse development. Genes Genet Syst. 2013;88(2):127-33.
  33. Han Z, Liu Q, Huang Z, Cui W, Tian Y, Yan W, Wu Q. Expression and imprinting analysis of AK044800, a transcript from the Dlk1-Dio3 imprinted gene cluster during mouse embryogenesis. Mol Cells. 2013 Apr;35(4):285-90.
  34. Xiao X, Li Z, Liu H, Su J, Wang F, Wu X, Liu H, Wu Q, Zhang Y. Genome-wide identification of Polycomb target genes in human embryonic stem cells. Gene. 2013 Apr 15;518(2):425-30.
  35. Qi L, Hongjuan H, Ning G, Zhengbin H, Yanjiang X, Tiebo Z, Zhijun H, Qiong W. miR-370 is stage-specifically expressed during mouse embryonic development and regulates Dnmt3a. FEBS Lett. 2013 Mar 18;587(6):775-81.
  36. Su J, Yan H, Wei Y, Liu H, Liu H, Wang F, Lv J, Wu Q, Zhang Y. CpG_MPs: identification of CpG methylation patterns of genomic regions from high-throughput bisulfite sequencing data. Nucleic Acids Res. 2013 Jan 7;41(1):e4.
  37. Yanjiang X, Hongjuan H, Tiantian G, Yan Z, Zhijun H, Qiong W. Expression patterns of ubiquitin conjugating enzyme UbcM2 during mouse embryonic development. Gene Expr. 2012;15(4):163-70.
  38. Gu T, He H, Zhang Y, Han Z, Hou G, Zeng T, Liu Q, Wu Q. Trmt112 gene expression in mouse embryonic development. Acta Histochem Cytochem. 2012 Apr 26;45(2):113-9.
  39. Zhang FW, Han ZB, Deng CY, He HJ, Wu Q. Conservation of genomic imprinting at the NDN, MAGEL2 and MEST loci in pigs. Genes Genet Syst. 2012;87(1):53-8.
  40. Yang L, Chen JH, Lv J, Wu Q, Xu T, Zhang H, Liu QH, Yang HK. Rice protein improves adiposity, body weight and reduces lipids level in rats through modification of triglyceride metabolism. Lipids Health Dis. 2012 Feb 13;11:24.
  41. Xu Q, Zhang F, He H, Xu S, Li K, Liu S, Li Y, Wu Q. Expression profile of mouse Mterfd2, a novel component of the mitochondrial transcription termination factor (MTERF) family. Genes Genet Syst. 2011;86(4):269-75.
  42. Han Z, He H, Zhang F, Huang Z, Liu Z, Jiang H, Wu Q. Spatiotemporal expression pattern of Mirg, an imprinted non-coding gene, during mouse embryogenesis. J Mol Histol. 2012 Feb;43(1):1-8.
  43. Gu T, He H, Han Z, Zeng T, Huang Z, Liu Q, Gu N, Chen Y, Sugimoto K, Jiang H, Wu Q. Expression of macro non-coding RNAs Meg8 and Irm in mouse embryonic development. Acta Histochem. 2012 Jul;114(4):392-9.
  44. Su J, Shao X, Liu H, Liu S, Wu Q, Zhang Y. Genome-wide dynamic changes of DNA methylation of repetitive elements in human embryonic stem cells and fetal fibroblasts. Genomics. 2012 Jan;99(1):10-7.
  45. Lv J, Liu H, Su J, Wu X, Liu H, Li B, Xiao X, Wang F, Wu Q, Zhang Y. DiseaseMeth: a human disease methylation database. Nucleic Acids Res. 2012 Jan;40(Database issue):D1030-5.
  46. Zhang FW, Zeng TB, Han ZB, He HJ, Chen Y, Gu N, Jiang HJ, Wu Q. Imprinting and expression analysis of a non-coding RNA gene in the mouse Dlk1-Dio3 domain. J Mol Histol. 2011 Aug;42(4):333-9.
  47. Zhang FW, Deng CY, He HJ, Gu N, Han ZB, Chen Y, Wu Q. Molecular cloning, mRNA expression and imprinting status of PEG3, NAP1L5 and PPP1R9A genes in pig. Genes Genet Syst. 2011;86(1):47-52.
  48. Zhang Y, Liu H, Lv J, Xiao X, Zhu J, Liu X, Su J, Li X, Wu Q, Wang F, Cui Y. QDMR: a quantitative method for identification of differentially methylated regions by entropy. Nucleic Acids Res. 2011 May;39(9):e58.
  49. Yan C, Hongjuan H, Yanjiang X, Zhengbin H, Kai L, Fengwei Z, Jing H, Qiong W. Expression patterns of imprinted gene Inpp5f-v3 during mouse brain development. J Mol Histol. 2011 Apr;42(2):167-73.
  50. Zeng F, Tian Y, Shi S, Wu Q, Liu S, Zheng H, Yue L, Li Y. Identification of mouse MARVELD1 as a microtubule associated protein that inhibits cell cycle progression and migration. Mol Cells. 2011 Mar;31(3):267-74.
  51. Gu T, He H, Xing Y, Liu Q, Gu N, Kenkichi S, Jiang H, Wu Q. Expression of non-coding RNA AB063319 derived from Rian gene during mouse development. J Mol Histol. 2011 Apr;42(2):105-12.
  52. Kawahara M, Wu Q, Kono T. Involvement of insulin-like growth factor 2 in angiogenic factor transcription in Bi-maternal mouse conceptuses. J Reprod Dev. 2010 Feb;56(1):79-85.
  53. Zhang Y, Lv J, Liu H, Zhu J, Su J, Wu Q, Qi Y, Wang F, Li X. HHMD: the human histone modification database. Nucleic Acids Res. 2010 Jan;38(Database issue):D149-54.
  54. Gao M, Liu Q, Zhang F, Han Z, Gu T, Tian W, Chen Y, Wu Q. Conserved expression of the PRELI domain containing 2 gene (Prelid2) during mid-later-gestation mouse embryogenesis. J Mol Histol. 2009 Jun;40(3):227-33.
  55. Liu Q, Wang Y, Chen Y, Zhang FW, Gu TT, Qu YP, Yue L, Wu Q. [The expression analysis of Grb10 during mouse embryonic development]. Yi Chuan. 2009 Jul;31(7):732-40. Chinese.
  56. Wu Q, Kawahara M, Kono T. Synergistic role of Igf2 and Dlk1 in fetal liver development and hematopoiesis in bi-maternal mice. J Reprod Dev. 2008 Jun;54(3):177-82.
  57. Kawahara M, Wu Q, Ferguson-Smith AC, Kono T. Appropriate expression of imprinted genes on mouse chromosome 12 extends development of bi-maternal embryos to term. FEBS Lett. 2007 Nov 13;581(27):5178-84.
  58. Kawahara M, Wu Q, Takahashi N, Morita S, Yamada K, Ito M, Ferguson-Smith AC, Kono T. High-frequency generation of viable mice from engineered bi-maternal embryos. Nat Biotechnol. 2007 Sep;25(9):1045-50.
  59. Ogawa H, Wu Q, Komiyama J, Obata Y, Kono T. Disruption of parental-specific expression of imprinted genes in uniparental fetuses. FEBS Lett. 2006 Oct 2;580(22):5377-84.
  60. Kawahara M, Wu Q, Yaguchi Y, Ferguson-Smith AC, Kono T. Complementary roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse placentation. Hum Mol Genet. 2006 Oct 1;15(19):2869-79.
  61. Kono T, Kawahara M, Wu Q, Hiura H, Obata Y. Paternal dual barrier by Ifg2-H19 and Dlk1-Gtl2 to parthenogenesis in mice. Ernst Schering Res Found Workshop. 2006;(60):23-33.
  62. Wu Q, Kumagai T, Kawahara M, Ogawa H, Hiura H, Obata Y, Takano R, Kono T. Regulated expression of two sets of paternally imprinted genes is necessary for mouse parthenogenetic development to term. Reproduction. 2006 Mar;131(3):481-8.
  63. Kono T, Obata Y, Wu Q, Niwa K, Ono Y, Yamamoto Y, Park ES, Seo JS, Ogawa H. Birth of parthenogenetic mice that can develop to adulthood. Nature. 2004 Apr 22;428(6985):860-4.
  64. 侯静，孔凡志，高卓，韩正滨，张凤伟，吴琼*, 陈岩. 印记基因Inpp5f-v3的片段克隆及其在小鼠胚胎发育中的表达.《生物技术通讯》2009 (6):789-791
  65. 孔凡志, 潘露露, 候静, 高卓, 高梦雅, 张凤伟, 陈岩, 吴琼*. 对与小鼠胚胎发育相关的印记基因Mcts2的研究. 《现代生物医学进展》2009 (13):2404-2406

 

 

承担主要科研项目

 

microRNA gene Mirg的表达与调控机制的研究(LC08C05)黑龙江省留学回国人员科技项目择优资助资金 2008年10月 2011年10月 负责人

教育部留学归国人员科研启动基金(GFEQ24403001)  负责人

mir-341、mir-1188、mir-370在小鼠发育中的表达与调控机制的研究(30971645) 国家自然科学基金面上项目 2010年1月-2012年12月 负责人

印记基因的机器学习方法预测及候选印记基因的实验鉴定及功能分析(31171383/C120105) 国家自然科学基金面上项目 2012年1月-2015年12月 负责人